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human pulmonary arterial ecs paecs  (ATCC)


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    ATCC human pulmonary arterial ecs paecs
    Human Pulmonary Arterial Ecs Paecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+arterial+ecs+paecs/Primary+Pulmonary+Artery+Endothelial+Cells%3B+Normal%2C+Human/10__1161_slash_jaha__124__034726-95-7-14
    Average 99 stars, based on 103 article reviews
    human pulmonary arterial ecs paecs - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Staining:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Western Blot:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    MANN-WHITNEY:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Expressing:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Control:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Marker:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Incubation:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Negative Control:

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).

    Article Title: Loss of Type 2 Bone Morphogenetic Protein Receptor Activates NOD‐Like Receptor Family Protein 3/Gasdermin E‐Mediated Pyroptosis in Pulmonary Arterial Hypertension
    Article Snippet: The cells used in the experiment are human pulmonary arterial ECs (PAECs) purchased from American Type Culture Collection.. The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).The cells were cultured at 37 °C under 5% CO 2 in DMEM F12 containing 10% fetal bovine serum (Hyclone, USA).



    Similar Products

    human pulmonary arterial ecs paecs  (ATCC)
    99
    ATCC human pulmonary arterial ecs paecs
    Human Pulmonary Arterial Ecs Paecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+arterial+ecs+paecs/Primary+Pulmonary+Artery+Endothelial+Cells%3B+Normal%2C+Human/10__1161_slash_jaha__124__034726-95-7-14
    Average 99 stars, based on 1 article reviews
    human pulmonary arterial ecs paecs - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    control human pulmonary artery ecs paecs  (PromoCell)
    95
    PromoCell control human pulmonary artery ecs paecs
    ( A ) Co-immunofluorescence staining of PCNA and Nestin reveals multiple Nestin + PCNA + cells (arrow) in the intima of a concentric lesion from a PAH patient. In contrast, no Nestin + PCNA + cells were detected in the intima of a pulmonary artery from a control subject. Scale bars: 50 μm (overview), 25 μm (detail images). For PAH, the image on the left shows an overview of the blood vessel, whereas the images in the middle demonstrate orthogonal views of z-stacks from the area indicate by a dotted box. The image on the right is an intensity projection of the whole z-stack. ( B ) Transient Nestin overexpression in rat lung <t>ECs</t> 72h after adenoviral transduction (qRT-PCR). β2-microglobulin ( B2m ) was used as housekeeping gene. n = 6 per group. ( C-D ) Increased BrdU incorporation over 4 h following Nestin overexpression in rat lung ECs (72h after adenovirus inoculation). (C) Representative histograms and (D) quantification. n = 6 per group. ( E ) Transient Nestin overexpression (qRT-PCR) in human control <t>PAECs</t> 72h after adenovirus transduction (qRT-PCR). TATA-binding protein ( TBP ) was used as housekeeping gene. (n = 4 per group). ( F ) qRT-PCR of MKI67 (Ki-67, a proliferation marker) and CXCL12 in hPAECs 72h after AdNES or AdDL70 treatment. n = 4 per group. All data shown as mean+SEM. * P <0.05, ** P <0.01 (Mann-Whitney). ( G ) Representative Western blots demonstrating the transgenic Nestin protein expression and elevated CXCL12 protein expression in PAECs 72h after adenoviral transfection with AdNES vs. AdDL70. β-actin was used as loading control.
    Control Human Pulmonary Artery Ecs Paecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+arterial+ecs+paecs/Human+Pulmonary+Artery+Endothelial+Cells/pmc06422269-94-0-23
    Average 95 stars, based on 1 article reviews
    control human pulmonary artery ecs paecs - by Bioz Stars, 2026-09
    95/100 stars
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    Image Search Results


    ( A ) Co-immunofluorescence staining of PCNA and Nestin reveals multiple Nestin + PCNA + cells (arrow) in the intima of a concentric lesion from a PAH patient. In contrast, no Nestin + PCNA + cells were detected in the intima of a pulmonary artery from a control subject. Scale bars: 50 μm (overview), 25 μm (detail images). For PAH, the image on the left shows an overview of the blood vessel, whereas the images in the middle demonstrate orthogonal views of z-stacks from the area indicate by a dotted box. The image on the right is an intensity projection of the whole z-stack. ( B ) Transient Nestin overexpression in rat lung ECs 72h after adenoviral transduction (qRT-PCR). β2-microglobulin ( B2m ) was used as housekeeping gene. n = 6 per group. ( C-D ) Increased BrdU incorporation over 4 h following Nestin overexpression in rat lung ECs (72h after adenovirus inoculation). (C) Representative histograms and (D) quantification. n = 6 per group. ( E ) Transient Nestin overexpression (qRT-PCR) in human control PAECs 72h after adenovirus transduction (qRT-PCR). TATA-binding protein ( TBP ) was used as housekeeping gene. (n = 4 per group). ( F ) qRT-PCR of MKI67 (Ki-67, a proliferation marker) and CXCL12 in hPAECs 72h after AdNES or AdDL70 treatment. n = 4 per group. All data shown as mean+SEM. * P <0.05, ** P <0.01 (Mann-Whitney). ( G ) Representative Western blots demonstrating the transgenic Nestin protein expression and elevated CXCL12 protein expression in PAECs 72h after adenoviral transfection with AdNES vs. AdDL70. β-actin was used as loading control.

    Journal: PLoS ONE

    Article Title: Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension

    doi: 10.1371/journal.pone.0213890

    Figure Lengend Snippet: ( A ) Co-immunofluorescence staining of PCNA and Nestin reveals multiple Nestin + PCNA + cells (arrow) in the intima of a concentric lesion from a PAH patient. In contrast, no Nestin + PCNA + cells were detected in the intima of a pulmonary artery from a control subject. Scale bars: 50 μm (overview), 25 μm (detail images). For PAH, the image on the left shows an overview of the blood vessel, whereas the images in the middle demonstrate orthogonal views of z-stacks from the area indicate by a dotted box. The image on the right is an intensity projection of the whole z-stack. ( B ) Transient Nestin overexpression in rat lung ECs 72h after adenoviral transduction (qRT-PCR). β2-microglobulin ( B2m ) was used as housekeeping gene. n = 6 per group. ( C-D ) Increased BrdU incorporation over 4 h following Nestin overexpression in rat lung ECs (72h after adenovirus inoculation). (C) Representative histograms and (D) quantification. n = 6 per group. ( E ) Transient Nestin overexpression (qRT-PCR) in human control PAECs 72h after adenovirus transduction (qRT-PCR). TATA-binding protein ( TBP ) was used as housekeeping gene. (n = 4 per group). ( F ) qRT-PCR of MKI67 (Ki-67, a proliferation marker) and CXCL12 in hPAECs 72h after AdNES or AdDL70 treatment. n = 4 per group. All data shown as mean+SEM. * P <0.05, ** P <0.01 (Mann-Whitney). ( G ) Representative Western blots demonstrating the transgenic Nestin protein expression and elevated CXCL12 protein expression in PAECs 72h after adenoviral transfection with AdNES vs. AdDL70. β-actin was used as loading control.

    Article Snippet: Control human pulmonary artery ECs (PAECs) were obtained from the Pulmonary Hypertension Breakthrough Initiative (PHBI) and cultured in endothelial growth medium 2 (EGM-2, Promocell, Heidelberg, Germany).

    Techniques: Immunofluorescence, Staining, Over Expression, Transduction, Quantitative RT-PCR, BrdU Incorporation Assay, Binding Assay, Marker, MANN-WHITNEY, Western Blot, Transgenic Assay, Expressing, Transfection